|
| The Molecular
Genetics laboratory offers the latest technology in Gene Expression Analysis.
We offers quantitative gene expression studies of nearly any gene. Our Gene expression
Analysis Unit has the most advanced techniques in this field, such as the Bioanalyzer
2100 of Agilent Technologies and the 7000 Sequence Detection System of Applied
Biosystems. |
| SERVICES |
| Due to the
specific demands of each project, pricing will be calculated individually. Please,
contact our commercial department for a customised free quotation.
|
| Lab-on-a-chip
Technology: |
| • Total and messenger RNA quantification and quality
parameters calculation of scanty biological samples |
| - 12 samples (minimum
25 ng / µl) : |
| - 11 samples (minimum 0,2 ng
/ µl) : |
| • DNA fragments and/or PCR products up to 1000
bp. (minimum 0,5 ng / µl) detection and quantification.
|
| (Maximum salt concentration: 25mM KCl/NaCl
and 15 mM MgCl2) |
|
 |
| Quantitative
PCR (Real Time PCR): |
| • Amplicon detection per sample per in triplicate: |
| - From cDNA: |
| - From RNA: |
| - From cells: |
| - From biopsies: |
| • Special projects |
| • Specific primers design and request |
|
|
| PROTOCOL
DELIVERY |
| The customer
must send the samples with the attached form completely filled and in the following
conditions:
• RNA --> Dry ice
• cDNA --> 4ºC
• Biopsies or cells --> Dry ice
Dominion Pharmakine recommends that primers are sent at a minimum concentration
of 20 µM with the samples at the time of ordering the quantitative PCR service.
Otherwise, specific primers design and request will be invoiced. |
|
| RESULTS
|
| Lab-on-a-chip:
Electropherogram images and resultant numeric data will be sent in two or five
working days from the reception of the sample.
RT-PCR: Amplification curves images and numeric data
will be sent in three to five working days (cDNA), six days (RNA) and nine days
(cell and tissue biopsies). |
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