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| The Cellular Biology Laboratory
offers the technology of Flow Cytometry, that can be used to assess the immunophenotype
of cell types, specific cell receptor(s), expression of surface and intracellular
antigens, etc … Our Flow Cytometry Unit has the Becton Dickinson FACS-Vantage
which is equipped with an Argon Laser with UV beam output in parallel with a 488nm
beam. It can detect several parameters in analysis or sorting mode: Forward scatter,
Side scatter and fluorescent dyes, such as FITC, PE, Percp or Percp-Cy5.5. It
also has an Automatic Cell Deposition Unit (ACDU) that sorts a predefined number
of cells into individual wells of a 96 or 24-well microtiter plate. |
| SERVICES
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| Measurement of cellular
parameters with a wide range of fluorescent markers |
| Available technologies: |
| • Apoptosis |
| • Proliferation |
| • Intracellular Gluthatione |
| • Cells viability |
| • Cell Cycle |
| • Stem cells (umbilical cord cells and peripheral
blood, cryopreserved and fresh, and from bone marrow) |
| • Phagocytosis (monocytes and granulocytes) |
| • Intracellular Cox-1/Cox-2 |
| • Basophil activation |
|
| High-speed cell sorting
with different expression antigens in the surface |
| PRICES |
| Due to the specific demands
of each project, pricing will be calculated individually. Please, contact our
Commercial Department for a customized free quotation. |
| PROTOCOL DELIVERY |
| The customer must send
the samples in PBS with the attached form completely filled. |
| RESULTS |
| Results of the Flow Cytometry
Service will consist in images with a spread-sheet as the customer request. |
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