Different hepatic cell types are isolated from male Sprague-Dawley rats following standard enzymatic perfusion and differential centrifugation techniques. Freshly isolated cells are then washed in PBS and fixed immediately in 4% formaldehyde for 1 hour. Cells are then spun down and carefully resuspended in pre-warmed 1% agarose (an inert material) and quickly cooled down on ice. Once cells are embedded in agarose they are again fixed in 4% formaldehyde over-night and processed as a piece of tissue in an automated tissue processor. Cells are embedded in paraffin blocks that will serve as donors and 1.5 mm cores are punched from the different cell types to build up the recipient block cell array.

Applications:

This organ-specific cell model offers the possibility to perform a variety of protein expression hepatic studies such as antibody screening using immunocytochemical techniques as well as in situ hybridization studies.

Array distribution:

Product Characteristics

Total array spots: 12

Array spot diameter: 1,5 mm

Tissue fixative: Zinc

Product Reference: DPK-CATS-01-R

Data Sheet

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